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KMID : 0525720160210010032
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Journal of Chitin and Chitosan
2016 Volume.21 No. 1 p.32 ~ p.42
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Development of Chitosan Based Natural Antibiotics for Diarrheal Treatment 1. Effect on Curing Piglets with Enterotoxin and E. coli Type Diarrhea
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Noh Hye-Ji
Kim Hee-Kyong
Cho Hyang-Hyun
Koh Hong-Bum
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Abstract
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The purpose of this research is to investigate activation of antibiotics toward livestock disease E. coli causing clinical disease and to apply OCHT to animal medicine for piglets diarrhea, by using OCHT made by raw material of chitosan. To solve the problem, after the production of OCHT controlled for the superior antibiotics, we selected OCHT with optimal physical state according to the laboratorial anitibiotics test. Lastly,the chosen OCHT is tried to be tested toward the piglets with diarrhea.Firstly, for the production of OCHT containing the optimized antibiotics, 3 types of OCHT which is divided according to the possession of molecule scope from oligosaccharids 1 to 6 (OCHT-1), 7-14 oligosaccharides range (OCHT-2), and 1-16 oligosaccharids range (OCHT-3) after disassembling raw material Chitosan with 96% of DAC into Chitosanase is prepared. And the OCHT is used for testing validity of laboratory and field. After the comparison among the laboratorial antibiosis toward the 26 types of E. coli based on different blood serum seperated form piglets with diarrhea. Therefore, OCHT-3 is finally chosen for the manufacturing convenience for the later product manufacture.In the concentration scope between 0.0001~0.5% of chosen OCHT-3, the density of E. coli of OCHT-3 significantly decreased when it is touched by E.coli type, in 1 hours regardless of density. After 12~24 hours, no gathering of E. coli was shown. (p<0.01). For the field applicability test on piglets with diarrhea toward OCHT-3, the assassement on the curing effect had been done 3 times a day (morning, noon and evening) for 7 days through the oral injection. It took maximum 4 days for treatment and the amount of OCHT for curing is minimum 2 mg to maximum 7.5 mg. Moreover, the dose for prevention is within 0.0001~1% (p<0.01). Therefore, OCHT is proved to be a natural antibiotics showing superior antibacterial and curing effect, regardless of the type of E. coli which shows differences on synthesized antibiotics tolerable germ and intestine toxic (p<0.01). In conclusition, OCHT (Product Name: EPECTROL-3) is a natural polysaccharose not showing the negative point of synthesized antibiotics as tolerance. In terms of antibiotics, OCHT contains selective molecule amount and physical properties. Also, OCHT was expected to be a natural antibiotics which can be applied to the stock raising industry.
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KEYWORD
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Chitosan-oligosaccharide, Piglet, E. coli, Antibacterial activity, Curative effect
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